From Comaiwiki

(A Sample List of 24 well-tested barcodes)
Line 3: Line 3:
  
 
These barcodes and associated oligonucleotides were generated using our [http://comailab.genomecenter.ucdavis.edu/index.php/Barcode_generator barcode generator].  For more background information on barcoding, see [http://comailab.genomecenter.ucdavis.edu/index.php/Video our instructional videos on Illumina Multiplexing].
 
These barcodes and associated oligonucleotides were generated using our [http://comailab.genomecenter.ucdavis.edu/index.php/Barcode_generator barcode generator].  For more background information on barcoding, see [http://comailab.genomecenter.ucdavis.edu/index.php/Video our instructional videos on Illumina Multiplexing].
 +
 +
 +
Barcodes of length 5 with a distance of 3 bases
 +
chosen for the adapter-barcode set on Oct 17 2009.
 +
index of barcodes:
 +
 +
>adA2_aaccc
 +
>adA2_acacg
 +
>adA2_accta
 +
>adA2_actac
 +
>adA2_aggtt
 +
>adA2_ccatc
 +
>adA2_cctct
 +
>adA2_ctagt
 +
>adA2_ctctg
 +
>adA2_gagta
 +
>adA2_gatgt
 +
>adA2_gcaga
 +
>adA2_gccat
 +
>adA2_ggtaa
 +
>adA2_gtcgc
 +
>adA2_gtgag
 +
>adA2_taagc
 +
>adA2_tacag
 +
>adA2_tagct
 +
>adA2_tcgaa
 +
>adA2_tctgg
 +
>adA2_tgatg
 +
>adA2_tgcgt
 +
>adA2_tttcc
 +
 +
List of oligonucleotides
 +
 +
Important: adA is 5' phosphorylated!
 +
>adA2_aaccc
 +
aacccAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
>adB2_aaccc
 +
ACACTCTTTCCCTACACGACGCTCTTCCGATCTgggttT
 +
 +
>adA2_acacg
 +
acacgAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
>adB2_acacg
 +
ACACTCTTTCCCTACACGACGCTCTTCCGATCTcgtgtT
 +
 +
>adA2_accta
 +
acctaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
>adB2_accta
 +
ACACTCTTTCCCTACACGACGCTCTTCCGATCTtaggtT
 +
 +
>adA2_actac
 +
actacAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
>adB2_actac
 +
ACACTCTTTCCCTACACGACGCTCTTCCGATCTgtagtT
 +
 +
>adA2_aggtt
 +
aggttAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
>adB2_aggtt
 +
ACACTCTTTCCCTACACGACGCTCTTCCGATCTaacctT
 +
 +
>adA2_ccatc
 +
ccatcAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
>adB2_ccatc
 +
ACACTCTTTCCCTACACGACGCTCTTCCGATCTgatggT
 +
 +
>adA2_cctct
 +
cctctAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
>adB2_cctct
 +
ACACTCTTTCCCTACACGACGCTCTTCCGATCTagaggT
 +
 +
>adA2_ctagt
 +
ctagtAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
>adB2_ctagt
 +
ACACTCTTTCCCTACACGACGCTCTTCCGATCTactagT
 +
 +
>adA2_ctctg
 +
ctctgAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
>adB2_ctctg
 +
ACACTCTTTCCCTACACGACGCTCTTCCGATCTcagagT
 +
 +
>adA2_gagta
 +
gagtaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
>adB2_gagta
 +
ACACTCTTTCCCTACACGACGCTCTTCCGATCTtactcT
 +
 +
>adA2_gatgt
 +
gatgtAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
>adB2_gatgt
 +
ACACTCTTTCCCTACACGACGCTCTTCCGATCTacatcT
 +
 +
>adA2_gcaga
 +
gcagaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
>adB2_gcaga
 +
ACACTCTTTCCCTACACGACGCTCTTCCGATCTtctgcT
 +
 +
>adA2_gccat
 +
gccatAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
>adB2_gccat
 +
ACACTCTTTCCCTACACGACGCTCTTCCGATCTatggcT
 +
 +
>adA2_ggtaa
 +
ggtaaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
>adB2_ggtaa
 +
ACACTCTTTCCCTACACGACGCTCTTCCGATCTttaccT
 +
 +
>adA2_gtcgc
 +
gtcgcAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
>adB2_gtcgc
 +
ACACTCTTTCCCTACACGACGCTCTTCCGATCTgcgacT
 +
 +
>adA2_gtgag
 +
gtgagAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
>adB2_gtgag
 +
ACACTCTTTCCCTACACGACGCTCTTCCGATCTctcacT
 +
 +
>adA2_taagc
 +
taagcAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
>adB2_taagc
 +
ACACTCTTTCCCTACACGACGCTCTTCCGATCTgcttaT
 +
 +
>adA2_tacag
 +
tacagAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
>adB2_tacag
 +
ACACTCTTTCCCTACACGACGCTCTTCCGATCTctgtaT
 +
 +
>adA2_tagct
 +
tagctAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
>adB2_tagct
 +
ACACTCTTTCCCTACACGACGCTCTTCCGATCTagctaT
 +
 +
>adA2_tcgaa
 +
tcgaaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
>adB2_tcgaa
 +
ACACTCTTTCCCTACACGACGCTCTTCCGATCTttcgaT
 +
 +
>adA2_tctgg
 +
tctggAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
>adB2_tctgg
 +
ACACTCTTTCCCTACACGACGCTCTTCCGATCTccagaT
 +
 +
>adA2_tgatg
 +
tgatgAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
>adB2_tgatg
 +
ACACTCTTTCCCTACACGACGCTCTTCCGATCTcatcaT
 +
 +
>adA2_tgcgt
 +
tgcgtAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
>adB2_tgcgt
 +
ACACTCTTTCCCTACACGACGCTCTTCCGATCTacgcaT
 +
 +
>adA2_tttcc
 +
tttccAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
>adB2_tttcc
 +
ACACTCTTTCCCTACACGACGCTCTTCCGATCTggaaaT

Revision as of 11:00, 17 February 2011

A Sample List of 24 well-tested barcodes

These barcodes and associated oligonucleotides were generated using our barcode generator. For more background information on barcoding, see our instructional videos on Illumina Multiplexing.


Barcodes of length 5 with a distance of 3 bases chosen for the adapter-barcode set on Oct 17 2009. index of barcodes:

>adA2_aaccc >adA2_acacg >adA2_accta >adA2_actac >adA2_aggtt >adA2_ccatc >adA2_cctct >adA2_ctagt >adA2_ctctg >adA2_gagta >adA2_gatgt >adA2_gcaga >adA2_gccat >adA2_ggtaa >adA2_gtcgc >adA2_gtgag >adA2_taagc >adA2_tacag >adA2_tagct >adA2_tcgaa >adA2_tctgg >adA2_tgatg >adA2_tgcgt >adA2_tttcc

List of oligonucleotides

Important: adA is 5' phosphorylated! >adA2_aaccc aacccAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_aaccc ACACTCTTTCCCTACACGACGCTCTTCCGATCTgggttT

>adA2_acacg acacgAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_acacg ACACTCTTTCCCTACACGACGCTCTTCCGATCTcgtgtT

>adA2_accta acctaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_accta ACACTCTTTCCCTACACGACGCTCTTCCGATCTtaggtT

>adA2_actac actacAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_actac ACACTCTTTCCCTACACGACGCTCTTCCGATCTgtagtT

>adA2_aggtt aggttAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_aggtt ACACTCTTTCCCTACACGACGCTCTTCCGATCTaacctT

>adA2_ccatc ccatcAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_ccatc ACACTCTTTCCCTACACGACGCTCTTCCGATCTgatggT

>adA2_cctct cctctAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_cctct ACACTCTTTCCCTACACGACGCTCTTCCGATCTagaggT

>adA2_ctagt ctagtAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_ctagt ACACTCTTTCCCTACACGACGCTCTTCCGATCTactagT

>adA2_ctctg ctctgAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_ctctg ACACTCTTTCCCTACACGACGCTCTTCCGATCTcagagT

>adA2_gagta gagtaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_gagta ACACTCTTTCCCTACACGACGCTCTTCCGATCTtactcT

>adA2_gatgt gatgtAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_gatgt ACACTCTTTCCCTACACGACGCTCTTCCGATCTacatcT

>adA2_gcaga gcagaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_gcaga ACACTCTTTCCCTACACGACGCTCTTCCGATCTtctgcT

>adA2_gccat gccatAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_gccat ACACTCTTTCCCTACACGACGCTCTTCCGATCTatggcT

>adA2_ggtaa ggtaaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_ggtaa ACACTCTTTCCCTACACGACGCTCTTCCGATCTttaccT

>adA2_gtcgc gtcgcAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_gtcgc ACACTCTTTCCCTACACGACGCTCTTCCGATCTgcgacT

>adA2_gtgag gtgagAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_gtgag ACACTCTTTCCCTACACGACGCTCTTCCGATCTctcacT

>adA2_taagc taagcAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_taagc ACACTCTTTCCCTACACGACGCTCTTCCGATCTgcttaT

>adA2_tacag tacagAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_tacag ACACTCTTTCCCTACACGACGCTCTTCCGATCTctgtaT

>adA2_tagct tagctAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_tagct ACACTCTTTCCCTACACGACGCTCTTCCGATCTagctaT

>adA2_tcgaa tcgaaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_tcgaa ACACTCTTTCCCTACACGACGCTCTTCCGATCTttcgaT

>adA2_tctgg tctggAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_tctgg ACACTCTTTCCCTACACGACGCTCTTCCGATCTccagaT

>adA2_tgatg tgatgAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_tgatg ACACTCTTTCCCTACACGACGCTCTTCCGATCTcatcaT

>adA2_tgcgt tgcgtAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_tgcgt ACACTCTTTCCCTACACGACGCTCTTCCGATCTacgcaT

>adA2_tttcc tttccAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_tttcc ACACTCTTTCCCTACACGACGCTCTTCCGATCTggaaaT

Personal tools