From Comaiwiki

Line 9: Line 9:
  
 
>adA2_aaccc
 
>adA2_aaccc
 +
 
>adA2_acacg
 
>adA2_acacg
  
Line 57: Line 58:
 
<strong>List of oligonucleotides</strong>
 
<strong>List of oligonucleotides</strong>
  
Important: adA is 5' phosphorylated!
+
<h2>Important: adA is 5' phosphorylated!!!!</h2>
 +
 
 
>adA2_aaccc
 
>adA2_aaccc
 
aacccAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 
aacccAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
 
>adB2_aaccc
 
>adB2_aaccc
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTgggttT
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTgggttT

Revision as of 11:03, 17 February 2011

A Sample List of 24 well-tested barcodes

These barcodes and associated oligonucleotides were generated using our barcode generator. For more background information on barcoding, see our instructional videos on Illumina Multiplexing.


Barcodes of length 5 with a distance of 3 bases chosen for the adapter-barcode set on Oct 17 2009.

Index of barcodes:

>adA2_aaccc

>adA2_acacg

>adA2_accta

>adA2_actac

>adA2_aggtt

>adA2_ccatc

>adA2_cctct

>adA2_ctagt

>adA2_ctctg

>adA2_gagta

>adA2_gatgt

>adA2_gcaga

>adA2_gccat

>adA2_ggtaa

>adA2_gtcgc

>adA2_gtgag

>adA2_taagc

>adA2_tacag

>adA2_tagct

>adA2_tcgaa

>adA2_tctgg

>adA2_tgatg

>adA2_tgcgt

>adA2_tttcc

List of oligonucleotides

Important: adA is 5' phosphorylated!!!!

>adA2_aaccc aacccAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_aaccc ACACTCTTTCCCTACACGACGCTCTTCCGATCTgggttT

>adA2_acacg acacgAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_acacg ACACTCTTTCCCTACACGACGCTCTTCCGATCTcgtgtT

>adA2_accta acctaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_accta ACACTCTTTCCCTACACGACGCTCTTCCGATCTtaggtT

>adA2_actac actacAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_actac ACACTCTTTCCCTACACGACGCTCTTCCGATCTgtagtT

>adA2_aggtt aggttAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_aggtt ACACTCTTTCCCTACACGACGCTCTTCCGATCTaacctT

>adA2_ccatc ccatcAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_ccatc ACACTCTTTCCCTACACGACGCTCTTCCGATCTgatggT

>adA2_cctct cctctAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_cctct ACACTCTTTCCCTACACGACGCTCTTCCGATCTagaggT

>adA2_ctagt ctagtAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_ctagt ACACTCTTTCCCTACACGACGCTCTTCCGATCTactagT

>adA2_ctctg ctctgAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_ctctg ACACTCTTTCCCTACACGACGCTCTTCCGATCTcagagT

>adA2_gagta gagtaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_gagta ACACTCTTTCCCTACACGACGCTCTTCCGATCTtactcT

>adA2_gatgt gatgtAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_gatgt ACACTCTTTCCCTACACGACGCTCTTCCGATCTacatcT

>adA2_gcaga gcagaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_gcaga ACACTCTTTCCCTACACGACGCTCTTCCGATCTtctgcT

>adA2_gccat gccatAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_gccat ACACTCTTTCCCTACACGACGCTCTTCCGATCTatggcT

>adA2_ggtaa ggtaaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_ggtaa ACACTCTTTCCCTACACGACGCTCTTCCGATCTttaccT

>adA2_gtcgc gtcgcAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_gtcgc ACACTCTTTCCCTACACGACGCTCTTCCGATCTgcgacT

>adA2_gtgag gtgagAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_gtgag ACACTCTTTCCCTACACGACGCTCTTCCGATCTctcacT

>adA2_taagc taagcAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_taagc ACACTCTTTCCCTACACGACGCTCTTCCGATCTgcttaT

>adA2_tacag tacagAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_tacag ACACTCTTTCCCTACACGACGCTCTTCCGATCTctgtaT

>adA2_tagct tagctAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_tagct ACACTCTTTCCCTACACGACGCTCTTCCGATCTagctaT

>adA2_tcgaa tcgaaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_tcgaa ACACTCTTTCCCTACACGACGCTCTTCCGATCTttcgaT

>adA2_tctgg tctggAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_tctgg ACACTCTTTCCCTACACGACGCTCTTCCGATCTccagaT

>adA2_tgatg tgatgAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_tgatg ACACTCTTTCCCTACACGACGCTCTTCCGATCTcatcaT

>adA2_tgcgt tgcgtAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_tgcgt ACACTCTTTCCCTACACGACGCTCTTCCGATCTacgcaT

>adA2_tttcc tttccAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_tttcc ACACTCTTTCCCTACACGACGCTCTTCCGATCTggaaaT

Personal tools