From Comaiwiki

Line 6: Line 6:
 
Barcodes of length 5 with a distance of 3 bases chosen for the adapter-barcode set on Oct 17 2009.
 
Barcodes of length 5 with a distance of 3 bases chosen for the adapter-barcode set on Oct 17 2009.
  
<strong>Index of barcodes:</strong>
+
<h2>Index of barcodes:</h2>
  
 
>adA2_aaccc
 
>adA2_aaccc
Line 56: Line 56:
 
>adA2_tttcc
 
>adA2_tttcc
  
<strong>List of oligonucleotides</strong>
+
<h2>List of oligonucleotides</h2>
  
<h2>Important: adA is 5' phosphorylated!!!!</h2>
+
<strong>Important Note: adA is 5' phosphorylated!!!!</strong>
  
 
>adA2_aaccc
 
>adA2_aaccc
Line 68: Line 68:
 
>adA2_acacg
 
>adA2_acacg
 
acacgAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 
acacgAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
 
>adB2_acacg
 
>adB2_acacg
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTcgtgtT
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTcgtgtT
Line 73: Line 74:
 
>adA2_accta
 
>adA2_accta
 
acctaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 
acctaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
 
>adB2_accta
 
>adB2_accta
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTtaggtT
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTtaggtT
Line 78: Line 80:
 
>adA2_actac
 
>adA2_actac
 
actacAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 
actacAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
 
>adB2_actac
 
>adB2_actac
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTgtagtT
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTgtagtT
Line 83: Line 86:
 
>adA2_aggtt
 
>adA2_aggtt
 
aggttAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 
aggttAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
 
>adB2_aggtt
 
>adB2_aggtt
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTaacctT
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTaacctT
Line 88: Line 92:
 
>adA2_ccatc
 
>adA2_ccatc
 
ccatcAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 
ccatcAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
 
>adB2_ccatc
 
>adB2_ccatc
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTgatggT
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTgatggT
Line 93: Line 98:
 
>adA2_cctct
 
>adA2_cctct
 
cctctAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 
cctctAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
 
>adB2_cctct
 
>adB2_cctct
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTagaggT
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTagaggT
Line 98: Line 104:
 
>adA2_ctagt
 
>adA2_ctagt
 
ctagtAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 
ctagtAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
 
>adB2_ctagt
 
>adB2_ctagt
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTactagT
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTactagT
Line 103: Line 110:
 
>adA2_ctctg
 
>adA2_ctctg
 
ctctgAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 
ctctgAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
 
>adB2_ctctg
 
>adB2_ctctg
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTcagagT
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTcagagT
Line 108: Line 116:
 
>adA2_gagta
 
>adA2_gagta
 
gagtaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 
gagtaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
 
>adB2_gagta
 
>adB2_gagta
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTtactcT
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTtactcT
Line 113: Line 122:
 
>adA2_gatgt
 
>adA2_gatgt
 
gatgtAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 
gatgtAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
 
>adB2_gatgt
 
>adB2_gatgt
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTacatcT
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTacatcT
Line 118: Line 128:
 
>adA2_gcaga
 
>adA2_gcaga
 
gcagaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 
gcagaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
 
>adB2_gcaga
 
>adB2_gcaga
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTtctgcT
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTtctgcT
Line 123: Line 134:
 
>adA2_gccat
 
>adA2_gccat
 
gccatAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 
gccatAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
 
>adB2_gccat
 
>adB2_gccat
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTatggcT
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTatggcT
Line 128: Line 140:
 
>adA2_ggtaa
 
>adA2_ggtaa
 
ggtaaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 
ggtaaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
 
>adB2_ggtaa
 
>adB2_ggtaa
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTttaccT
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTttaccT
Line 133: Line 146:
 
>adA2_gtcgc
 
>adA2_gtcgc
 
gtcgcAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 
gtcgcAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
 
>adB2_gtcgc
 
>adB2_gtcgc
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTgcgacT
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTgcgacT
Line 138: Line 152:
 
>adA2_gtgag
 
>adA2_gtgag
 
gtgagAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 
gtgagAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
 
>adB2_gtgag
 
>adB2_gtgag
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTctcacT
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTctcacT
Line 143: Line 158:
 
>adA2_taagc
 
>adA2_taagc
 
taagcAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 
taagcAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
 
>adB2_taagc
 
>adB2_taagc
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTgcttaT
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTgcttaT
Line 148: Line 164:
 
>adA2_tacag
 
>adA2_tacag
 
tacagAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 
tacagAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
 
>adB2_tacag
 
>adB2_tacag
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTctgtaT
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTctgtaT
Line 153: Line 170:
 
>adA2_tagct
 
>adA2_tagct
 
tagctAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 
tagctAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
 
>adB2_tagct
 
>adB2_tagct
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTagctaT
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTagctaT
Line 158: Line 176:
 
>adA2_tcgaa
 
>adA2_tcgaa
 
tcgaaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 
tcgaaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
 
>adB2_tcgaa
 
>adB2_tcgaa
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTttcgaT
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTttcgaT
Line 163: Line 182:
 
>adA2_tctgg
 
>adA2_tctgg
 
tctggAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 
tctggAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
 
>adB2_tctgg
 
>adB2_tctgg
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTccagaT
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTccagaT
Line 168: Line 188:
 
>adA2_tgatg
 
>adA2_tgatg
 
tgatgAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 
tgatgAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
 
>adB2_tgatg
 
>adB2_tgatg
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTcatcaT
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTcatcaT
Line 173: Line 194:
 
>adA2_tgcgt
 
>adA2_tgcgt
 
tgcgtAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 
tgcgtAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
 
>adB2_tgcgt
 
>adB2_tgcgt
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTacgcaT
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTacgcaT
Line 178: Line 200:
 
>adA2_tttcc
 
>adA2_tttcc
 
tttccAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 
tttccAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
 +
 
>adB2_tttcc
 
>adB2_tttcc
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTggaaaT
 
ACACTCTTTCCCTACACGACGCTCTTCCGATCTggaaaT

Revision as of 11:06, 17 February 2011

A Sample List of 24 well-tested barcodes

These barcodes and associated oligonucleotides were generated using our barcode generator. For more background information on barcoding, see our instructional videos on Illumina Multiplexing.


Barcodes of length 5 with a distance of 3 bases chosen for the adapter-barcode set on Oct 17 2009.

Index of barcodes:

>adA2_aaccc

>adA2_acacg

>adA2_accta

>adA2_actac

>adA2_aggtt

>adA2_ccatc

>adA2_cctct

>adA2_ctagt

>adA2_ctctg

>adA2_gagta

>adA2_gatgt

>adA2_gcaga

>adA2_gccat

>adA2_ggtaa

>adA2_gtcgc

>adA2_gtgag

>adA2_taagc

>adA2_tacag

>adA2_tagct

>adA2_tcgaa

>adA2_tctgg

>adA2_tgatg

>adA2_tgcgt

>adA2_tttcc

List of oligonucleotides

Important Note: adA is 5' phosphorylated!!!!

>adA2_aaccc aacccAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_aaccc ACACTCTTTCCCTACACGACGCTCTTCCGATCTgggttT

>adA2_acacg acacgAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_acacg ACACTCTTTCCCTACACGACGCTCTTCCGATCTcgtgtT

>adA2_accta acctaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_accta ACACTCTTTCCCTACACGACGCTCTTCCGATCTtaggtT

>adA2_actac actacAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_actac ACACTCTTTCCCTACACGACGCTCTTCCGATCTgtagtT

>adA2_aggtt aggttAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_aggtt ACACTCTTTCCCTACACGACGCTCTTCCGATCTaacctT

>adA2_ccatc ccatcAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_ccatc ACACTCTTTCCCTACACGACGCTCTTCCGATCTgatggT

>adA2_cctct cctctAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_cctct ACACTCTTTCCCTACACGACGCTCTTCCGATCTagaggT

>adA2_ctagt ctagtAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_ctagt ACACTCTTTCCCTACACGACGCTCTTCCGATCTactagT

>adA2_ctctg ctctgAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_ctctg ACACTCTTTCCCTACACGACGCTCTTCCGATCTcagagT

>adA2_gagta gagtaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_gagta ACACTCTTTCCCTACACGACGCTCTTCCGATCTtactcT

>adA2_gatgt gatgtAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_gatgt ACACTCTTTCCCTACACGACGCTCTTCCGATCTacatcT

>adA2_gcaga gcagaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_gcaga ACACTCTTTCCCTACACGACGCTCTTCCGATCTtctgcT

>adA2_gccat gccatAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_gccat ACACTCTTTCCCTACACGACGCTCTTCCGATCTatggcT

>adA2_ggtaa ggtaaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_ggtaa ACACTCTTTCCCTACACGACGCTCTTCCGATCTttaccT

>adA2_gtcgc gtcgcAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_gtcgc ACACTCTTTCCCTACACGACGCTCTTCCGATCTgcgacT

>adA2_gtgag gtgagAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_gtgag ACACTCTTTCCCTACACGACGCTCTTCCGATCTctcacT

>adA2_taagc taagcAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_taagc ACACTCTTTCCCTACACGACGCTCTTCCGATCTgcttaT

>adA2_tacag tacagAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_tacag ACACTCTTTCCCTACACGACGCTCTTCCGATCTctgtaT

>adA2_tagct tagctAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_tagct ACACTCTTTCCCTACACGACGCTCTTCCGATCTagctaT

>adA2_tcgaa tcgaaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_tcgaa ACACTCTTTCCCTACACGACGCTCTTCCGATCTttcgaT

>adA2_tctgg tctggAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_tctgg ACACTCTTTCCCTACACGACGCTCTTCCGATCTccagaT

>adA2_tgatg tgatgAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_tgatg ACACTCTTTCCCTACACGACGCTCTTCCGATCTcatcaT

>adA2_tgcgt tgcgtAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_tgcgt ACACTCTTTCCCTACACGACGCTCTTCCGATCTacgcaT

>adA2_tttcc tttccAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_tttcc ACACTCTTTCCCTACACGACGCTCTTCCGATCTggaaaT

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