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A Sample List of 24 well-tested barcodes

These barcodes and associated oligonucleotides were generated using our barcode generator. For more background information on barcoding, see our instructional videos on Illumina Multiplexing.


Barcodes of length 5 with a distance of 3 bases chosen for the adapter-barcode set on Oct 17 2009.

Index of barcodes:

>adA2_aaccc >adA2_acacg >adA2_accta >adA2_actac >adA2_aggtt >adA2_ccatc >adA2_cctct >adA2_ctagt >adA2_ctctg >adA2_gagta >adA2_gatgt >adA2_gcaga >adA2_gccat >adA2_ggtaa >adA2_gtcgc >adA2_gtgag >adA2_taagc >adA2_tacag >adA2_tagct >adA2_tcgaa >adA2_tctgg >adA2_tgatg >adA2_tgcgt >adA2_tttcc

List of oligonucleotides

Important: adA is 5' phosphorylated! >adA2_aaccc aacccAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_aaccc ACACTCTTTCCCTACACGACGCTCTTCCGATCTgggttT

>adA2_acacg acacgAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_acacg ACACTCTTTCCCTACACGACGCTCTTCCGATCTcgtgtT

>adA2_accta acctaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_accta ACACTCTTTCCCTACACGACGCTCTTCCGATCTtaggtT

>adA2_actac actacAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_actac ACACTCTTTCCCTACACGACGCTCTTCCGATCTgtagtT

>adA2_aggtt aggttAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_aggtt ACACTCTTTCCCTACACGACGCTCTTCCGATCTaacctT

>adA2_ccatc ccatcAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_ccatc ACACTCTTTCCCTACACGACGCTCTTCCGATCTgatggT

>adA2_cctct cctctAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_cctct ACACTCTTTCCCTACACGACGCTCTTCCGATCTagaggT

>adA2_ctagt ctagtAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_ctagt ACACTCTTTCCCTACACGACGCTCTTCCGATCTactagT

>adA2_ctctg ctctgAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_ctctg ACACTCTTTCCCTACACGACGCTCTTCCGATCTcagagT

>adA2_gagta gagtaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_gagta ACACTCTTTCCCTACACGACGCTCTTCCGATCTtactcT

>adA2_gatgt gatgtAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_gatgt ACACTCTTTCCCTACACGACGCTCTTCCGATCTacatcT

>adA2_gcaga gcagaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_gcaga ACACTCTTTCCCTACACGACGCTCTTCCGATCTtctgcT

>adA2_gccat gccatAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_gccat ACACTCTTTCCCTACACGACGCTCTTCCGATCTatggcT

>adA2_ggtaa ggtaaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_ggtaa ACACTCTTTCCCTACACGACGCTCTTCCGATCTttaccT

>adA2_gtcgc gtcgcAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_gtcgc ACACTCTTTCCCTACACGACGCTCTTCCGATCTgcgacT

>adA2_gtgag gtgagAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_gtgag ACACTCTTTCCCTACACGACGCTCTTCCGATCTctcacT

>adA2_taagc taagcAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_taagc ACACTCTTTCCCTACACGACGCTCTTCCGATCTgcttaT

>adA2_tacag tacagAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_tacag ACACTCTTTCCCTACACGACGCTCTTCCGATCTctgtaT

>adA2_tagct tagctAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_tagct ACACTCTTTCCCTACACGACGCTCTTCCGATCTagctaT

>adA2_tcgaa tcgaaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_tcgaa ACACTCTTTCCCTACACGACGCTCTTCCGATCTttcgaT

>adA2_tctgg tctggAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_tctgg ACACTCTTTCCCTACACGACGCTCTTCCGATCTccagaT

>adA2_tgatg tgatgAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_tgatg ACACTCTTTCCCTACACGACGCTCTTCCGATCTcatcaT

>adA2_tgcgt tgcgtAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_tgcgt ACACTCTTTCCCTACACGACGCTCTTCCGATCTacgcaT

>adA2_tttcc tttccAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG >adB2_tttcc ACACTCTTTCCCTACACGACGCTCTTCCGATCTggaaaT

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