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A Sample List of 24 well-tested barcodes

These barcodes and associated oligonucleotides were generated using our barcode generator. For more background information on barcoding, see our instructional videos on Illumina Multiplexing.


Barcodes of length 5 with a distance of 3 bases chosen for the adapter-barcode set on Oct 17 2009.

Index of barcodes:

>adA2_aaccc

>adA2_acacg

>adA2_accta

>adA2_actac

>adA2_aggtt

>adA2_ccatc

>adA2_cctct

>adA2_ctagt

>adA2_ctctg

>adA2_gagta

>adA2_gatgt

>adA2_gcaga

>adA2_gccat

>adA2_ggtaa

>adA2_gtcgc

>adA2_gtgag

>adA2_taagc

>adA2_tacag

>adA2_tagct

>adA2_tcgaa

>adA2_tctgg

>adA2_tgatg

>adA2_tgcgt

>adA2_tttcc

List of oligonucleotides

Important Note: adA is 5' phosphorylated!!!!

>adA2_aaccc aacccAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_aaccc ACACTCTTTCCCTACACGACGCTCTTCCGATCTgggttT

>adA2_acacg acacgAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_acacg ACACTCTTTCCCTACACGACGCTCTTCCGATCTcgtgtT

>adA2_accta acctaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_accta ACACTCTTTCCCTACACGACGCTCTTCCGATCTtaggtT

>adA2_actac actacAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_actac ACACTCTTTCCCTACACGACGCTCTTCCGATCTgtagtT

>adA2_aggtt aggttAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_aggtt ACACTCTTTCCCTACACGACGCTCTTCCGATCTaacctT

>adA2_ccatc ccatcAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_ccatc ACACTCTTTCCCTACACGACGCTCTTCCGATCTgatggT

>adA2_cctct cctctAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_cctct ACACTCTTTCCCTACACGACGCTCTTCCGATCTagaggT

>adA2_ctagt ctagtAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_ctagt ACACTCTTTCCCTACACGACGCTCTTCCGATCTactagT

>adA2_ctctg ctctgAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_ctctg ACACTCTTTCCCTACACGACGCTCTTCCGATCTcagagT

>adA2_gagta gagtaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_gagta ACACTCTTTCCCTACACGACGCTCTTCCGATCTtactcT

>adA2_gatgt gatgtAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_gatgt ACACTCTTTCCCTACACGACGCTCTTCCGATCTacatcT

>adA2_gcaga gcagaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_gcaga ACACTCTTTCCCTACACGACGCTCTTCCGATCTtctgcT

>adA2_gccat gccatAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_gccat ACACTCTTTCCCTACACGACGCTCTTCCGATCTatggcT

>adA2_ggtaa ggtaaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_ggtaa ACACTCTTTCCCTACACGACGCTCTTCCGATCTttaccT

>adA2_gtcgc gtcgcAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_gtcgc ACACTCTTTCCCTACACGACGCTCTTCCGATCTgcgacT

>adA2_gtgag gtgagAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_gtgag ACACTCTTTCCCTACACGACGCTCTTCCGATCTctcacT

>adA2_taagc taagcAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_taagc ACACTCTTTCCCTACACGACGCTCTTCCGATCTgcttaT

>adA2_tacag tacagAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_tacag ACACTCTTTCCCTACACGACGCTCTTCCGATCTctgtaT

>adA2_tagct tagctAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_tagct ACACTCTTTCCCTACACGACGCTCTTCCGATCTagctaT

>adA2_tcgaa tcgaaAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_tcgaa ACACTCTTTCCCTACACGACGCTCTTCCGATCTttcgaT

>adA2_tctgg tctggAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_tctgg ACACTCTTTCCCTACACGACGCTCTTCCGATCTccagaT

>adA2_tgatg tgatgAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_tgatg ACACTCTTTCCCTACACGACGCTCTTCCGATCTcatcaT

>adA2_tgcgt tgcgtAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_tgcgt ACACTCTTTCCCTACACGACGCTCTTCCGATCTacgcaT

>adA2_tttcc tttccAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

>adB2_tttcc ACACTCTTTCCCTACACGACGCTCTTCCGATCTggaaaT

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