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(Analysis of next generation sequencing data: a introductory video tutorial)
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===[[Positions available]]===
We are recruiting paid and internship-based undergraduates. Click on the above link for more information.
===Recently published or in press===
===Recently published or in press===

Revision as of 14:06, 29 March 2011




The Comai Lab is in the Department of Plant Biology and the UC Davis Genome Center. One part of the laboratory studies how chromosome number and type affect gene regulation, development and genome evolution. Our model systems are Arabidopsis thaliana and rice. The other laboratory part is adapting next generation sequencing to a functional discovery tool called TILLING. We are developing methods and services to efficiently find mutations in genes of interest in rice, wheat, tomato, and tetraploid Arabidopsis. Click on the research links below to find out more.

Polyploidy, Heterosis, and TILLING



Pubmed report


Recently published or in press

Chr4 ih.png

Isabelle Henry et al. characterize the aneuploids of Arabidopsis. They conclude that multiple developmental phenotypes can be explained simply by the dosage of one or two chromosomes and are insensitive to the imbalance of others. They demonstrate that karyotyping can be easily accomplished by low-density sequencing with Illumina. Unexpectedly, the diploid progeny of trisomics are not fully wild-type but exhibit distinct alterations in development. The paper, entitled "Phenotypic consequences of aneuploidy in Arabidopsis thaliana", was published in the journal Genetics. The image above demonstrates that effect of a terminal translocation of chr 4 onto one copy of a trisomic chromosome.

TILLING workshops

A TILLING training workshop was held Aug 24 and 25 2009, and Aug 23 and 24 2010 in Davis. An additional one is planned for August 2011.

Analysis of next generation sequencing data: a introductory video tutorial


We offer instructional video tutorials on manipulating and analyzing datasets from next-generation sequencing. The target audience is biologists who might use these techniques but would like to perform some of the analysis themselves.

Personal tools