From Comaiwiki

Revision as of 13:08, 7 April 2013 by Root (Talk | contribs)

Logo.png

Contents

Introduction

Perennial, clonally propagated plants provide a challenge and an opportunity for functional gene discovery. Meiotic recombination-based genetic analysis and breeding are hampered by the long generation cycle. On the other hand, clonal propagation enables the production of many individuals with identical genotypes and economic exploitation of plants without the need for sexual reproduction. In collaboration with Andrew Groover at US Forest Service, Davis we are studying the effect of genomic copy number variation on poplar hybrid performance. We are studying both variation induced by hybridization and by ionizing radiation, the latter applied to pollen during interspecific hybridization.

Approach

Next generation sequencing-based genotyping and karyotyping will be used to characterize ploidy changes, deletions and possible rearrangements in Populus hybrids, including both F1 pedigrees and unrelated F1 individuals.

Poplar hybridization.png Variation in crosses between different species will be characterized genotypically and karyotypically
Poplar variation.png To enhance variation and test the contribution of smaller chromosomal regions to phenotype, γ−irradiation of pollen is used to induce deletions of varying size. The method produces germplasm enriched with genotypic and chromosomal dosage variation compared to traditional Populus hybrids. This germplasm will serve as a testbed for exploring correlations between chromosomal segments, their dosage and phenotypes, and to identify commercially-relevant genomic combinations.
[[image:|300px|]] rosa

Results

Funding

Our research is funded by the Department of Energy grant 201118510 entitled Creation of High-Precision Characterization of Novel Poplar Biomass Germplasm

Summary of DOE grant proposal

Personal tools